Author

Shane D. Gilligan-Steinberg

University of WashingtonORCID 0000-0001-9036-565XUnclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Research Focus:benchmarking· 1 Assay:bulk RNA-seq· 1 Research Focus:cell type annotation· 1 Research Focus:drug response / drug discovery· 1 Approach:genetic perturbation / CRISPR· 1 Research Focus:host-pathogen & immune response· 1

Frequent Co-Authors

Barry R. LutziD· 2 shared Aishwarya MageshiD· 1 shared Alexander K. BeaveriD· 1 shared Ayokunle O. OlanrewajuiD· 1 shared Bailin ZhangiD· 1 shared Cara BrainerdiD· 1 shared Cosette A. CraigiD· 1 shared Dharani Narayan MoorthyiD· 1 shared

Activity by Year

2024-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 17 mutual co-author links

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2 shared papersBioengineering

Barry R. LutzORCID

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3 papers

Recent·Most cited

BioengineeringbioRxivPreprintOct 12, 2026

Surfactant-Enhanced Hybridization Capture for Direct Sequence-Specific RNA Extraction from Plasma and Whole Blood

Wookyeom Kim, Shane D. Gilligan-Steinberg, Enos C. Kline…·Barry R. Lutz

This paper introduces a surfactant-based method for direct RNA extraction from plasma and whole blood, achieving high recovery of low-abundance RNA and outperforming existing silica-based kits.

DOI 10.64898/2026.10.06.757078

bulk RNA-seqexperimental assay

𝕏 PostBluesky

MicrobiologyNaturePeer-reviewedApr 8, 2026

DNA damage drives antigen diversification through mosaic VSG formation in Trypanosoma brucei

Jaclyn E. Smith, Kevin J. Wang, Erika Kennedy…·Monica R. Mugnier

The paper demonstrates that DNA damage induces VSG recombination in T. brucei, leading to antigenic variation and immune evasion.

9 citationstop 1%

experimental assaygenetic perturbation / CRISPR

𝕏 PostBluesky

BioengineeringAnalytical and Bioanalytical ChemistryPeer-reviewedOct 28, 2024

REverse transcriptase ACTivity (REACT) assay for point-of-care measurement of established and emerging antiretrovirals for HIV treatment and prevention

Cara Brainerd, Maya A. Singh, John A Tatka…·Ayokunle O. Olanrewaju

The paper introduces a rapid, portable assay called REACT to measure NNRTIs for HIV treatment and prevention, showing high accuracy in both buffer and plasma samples.

5 citations

experimental assaydrug response / drug discovery

𝕏 PostBluesky