A rapid HPLC-based method to determine NAD(P)(H) and NMN redox cofactor concentrations and ratios in microbes
Noor E van Wijk, Elisabeth C. M. van der Heijden, Javier M Hernández-Sancho…·Markus M.M. Bisschops
This paper introduces a rapid HPLC-based method to measure NAD(P)(H) and NMN redox cofactor concentrations and ratios in microbes, validated against a classical assay and applied to engineered E. coli and Pseudomonas putida.