Taylor L. Mighell

GPCR-MAPS: high-resolution functional and allosteric mapping of G protein-coupled receptor activation and bias

The paper introduces GPCR-MAPS, a method to generate high-resolution functional and allosteric maps of GPCRs, revealing modular architecture and novel allosteric pockets.

A pharmacological chaperone stabilizer rescues the expression of the vast majority of pathogenic variants in a G protein-coupled receptor

The paper tests a pharmacological chaperone's ability to rescue expression of destabilizing variants in the V2R gene, finding it effective for most variants.

SUNi mutagenesis: scalable and uniform nicking for efficient generation of variant libraries

The paper introduces SUNi mutagenesis, a method for generating variant libraries with high scalability and uniformity to enable MAVEs of gene families and genomes.

Cas12a-Capture: a novel, low-cost, and scalable method for targeted sequencing

The paper introduces Cas12a-Capture, a low-cost, scalable targeted sequencing method that reduces GC bias and improves variant calling accuracy.

An integrated deep mutational scanning approach provides clinical insights on PTEN genotype-phenotype relationships

This study combines deep mutational scanning data with clinical data to link PTEN variants to specific clinical outcomes and risk levels.

A saturation mutagenesis approach to understanding PTEN lipid phosphatase activity and genotype-phenotypes relationships

The paper uses a saturation mutagenesis approach in a yeast model to assess PTEN variants' impact on lipid phosphatase activity, identifying functional scores for 7,244 variants and reclassifying some ClinVar unclassified variants.