Deciphering cytokine-driven ADP-ribosylation signaling networks via Af1521-based mass spectrometry analysis of labile Glu/Asp-linkages
Sara Charlotte Buch-Larsen, Ivo Alexander Hendriks, Kyuto Tashiro, Jonas Damgaard Elsborg, Sergey Y. Vakhrushev, Jesper Velgaard Olsen, Bernhard Lüscher, Glen Liszczak, Ivan Ahel, Michael Lund Nielsen
The paper develops an MS-based workflow to detect Glu/Asp-ADPr modifications, identifies over 600 sites in cytokine-stimulated cells, and reveals residue-specific ADPr dynamics in immune signaling.
Nature Communications · May 27, 2026Peer-reviewedCell Biology