Bernhard Lüscher

Dimerization of human PARP15 is required for NAD+ binding and automodification

This study shows that dimerization of PARP15 is necessary for NAD+ binding and automodification, supported by structural and biochemical evidence.

Deciphering cytokine-driven ADP-ribosylation signaling networks via Af1521-based mass spectrometry analysis of labile Glu/Asp-linkages

The paper develops an MS-based workflow to detect Glu/Asp-ADPr modifications, identifies over 600 sites in cytokine-stimulated cells, and reveals residue-specific ADPr dynamics in immune signaling.

Reversing PROTAC-induced ASH2L degradation reactivates proliferation in senescent cells

The paper shows that reversing PROTAC-induced ASH2L degradation restores H3K4me3, gene expression, and proliferation in senescent cells.

Human PARPs modify RNA nucleobases in vitro and in cells

This study shows PARP10 and PARP15 modify RNA nucleobases in vitro and in cells, and TARG1 reverses these modifications.

Sequential deregulation of histone marks, chromatin accessibility and gene expression in response to PROTAC-induced degradation of ASH2L

This study shows that ASH2L degradation via PROTAC leads to sequential changes in histone marks, chromatin accessibility, and gene expression, revealing delayed downstream effects on transcription.

Systematic analysis of ADP-ribose detection reagents and optimisation of sample preparation to detect ADP-ribosylation in vitro and in cells