This study identifies SUGP1 as a key splicing factor in piRNA biogenesis in Bombyx mori, revealing its interaction with Y-box protein and dual role in piRNA maturation and transposon regulation.

bioRxiv · Oct 9, 2025 · PreprintMolecular Biology

High-throughput and genome-scale targeted mutagenesis using CRISPR in a non-model multicellular organism, Bombyx mori

Sanyuan Ma, Tong Zhang, Ruolin Wang, Pan Wang, Yue Liu, Jiasong Chang, Aoming Wang, Xinhui Lan, Le Sun, Hao Sun, Run Shi, Wei Lü, Dan Liu, Na Zhang, Wenbo Hu, , Qingyou Xia

The paper develops a CRISPR-based method to generate a large-scale mutant library in Bombyx mori, identifying genes involved in phenotypic changes and environmental tolerance.

Genome Research · Jan 1, 2024Peer-reviewedGenomics

Molecular atlas reveals the tri-sectional spinning mechanism of spider dragline silk

Wenbo Hu, Anqiang Jia, Sanyuan Ma, Guoqing Zhang, Zhaoyuan Wei, Fang Lü, Yongjiang Luo, Zhi‐Sheng Zhang, Jiahe Sun, Tianfang Yang, Tingting Xia, Qinhui Li, Ting Yao, Jiangyu Zheng, Zijie Jiang, , Yi Wang

The paper creates a molecular atlas of spider dragline silk production in Trichonephila clavata, revealing a tri-sectional biosynthesis mechanism and cell-type organization in the major ampullate gland.

Nature Communications · Feb 15, 2023Peer-reviewedGenomics

Programmable single and multiplex base-editing in Bombyx mori using RNA-guided cytidine deaminases

Yufeng Li, Sanyuan Ma, Le Sun, Tong Zhang, Jiasong Chang, Wei Lü, Xiaoxu Chen, Yue Liu, Xiaogang Wang, Run Shi, Ping Zhao, Qingyou Xia

This paper demonstrates the use of RNA-guided cytidine deaminases for programmable single and multiplex base-editing in Bombyx mori with high efficiency and low indel frequency.

G3 Genes Genomes Genetics · Mar 19, 2018Peer-reviewedGenetics