This study identifies oligodendrocytes as the major source of SERPINA3N in ischemic stroke and shows that antagonizing it improves neurological outcomes.

bioRxiv · Jul 29, 2026 · PreprintNeuroscience

The glycolytic reaction PGAM unexpectedly restrains Th17 pathogenicity and Th17-dependent autoimmunity

Chao Wang, Allon Wagner, Johannes Fessler, David DeTomaso, Sarah Zaghouani, Yulin Zhou, Kerry A. Pierce, Raymond A. Sobel, Clary B. Clish, Nir Yosef, Vijay Kumar Kuchroo

This study identifies PGAM as a negative regulator of Th17 pathogenicity using single-cell RNA-seq and metabolic flux analysis.

Cell Reports · Jun 1, 2025Peer-reviewedImmunology

In Silico Modeling of Metabolic State in Single Th17 Cells Reveals Novel Regulators of Inflammation and Autoimmunity

Allon Wagner, Chao Wang, Johannes Fessler, David DeTomaso, Julián Ávila-Pacheco, James Kaminski, Sarah Zaghouani, Elena Christian, Pratiksha I. Thakore, Brandon Schellhaass, Elliot H. Akama‐Garren, Kerry A. Pierce, Vasundhara Singh, Noga Ron‐Harel, Vivian Paraskevi Douglas, , Nir Yosef

The paper introduces Compass, an algorithm that uses single-cell RNA-Seq and flux balance analysis to characterize metabolic states in Th17 cells, revealing a metabolic switch and novel anti-inflammatory regulators.

Cell · Jul 2, 2021Peer-reviewedGenomics