This study characterizes the biosynthesis of captophorins, a class of Cu-associated peptidic thiooxazole metallophores, by analyzing MNIO enzymes and their products.

ACS Chemical Biology · Mar 12, 2026Peer-reviewedBiochemistry

Lantibiotic-producing bacteria impact microbiome resilience and colonization resistance

Cody G. Cole, Zhenrun Jerry Zhang, Shravan R. Dommaraju, Qiwen Dong, Rosemary L. Pope, Sophie S Son, Emma McSpadden, Che Woodson, Huaiying Lin, Nicholas P. Dylla, Ashley M. Sidebottom, Anitha Sundararajan, Douglas A. Mitchell, Eric G. Pamer

This study shows lantibiotic-producing bacteria reduce microbiome resilience and increase susceptibility to infections after antibiotic treatment.

Cell Host & Microbe · Dec 1, 2025Peer-reviewedMicrobiology

A versatile enzymatic pathway for modification of peptide C-termini

Shravan R. Dommaraju, Sanath K. Kandy, Hengqian Ren, Dominic P. Luciano, Shogo Fujiki, David Šarlah, Huimin Zhao, Jonathan R. Chekan, Douglas A. Mitchell

The paper reconstitutes daptide biosynthesis in vitro to reveal enzymatic requirements and demonstrates broad substrate tolerance for modifying peptide C-termini.

ACS Central Science · Sep 20, 2025Peer-reviewedBiochemistry

Computationally guided exploration of borosin biosynthetic strategies

Aileen R. Lee, Riley S. Carter, Aman S. Imani, Shravan R. Dommaraju, Graham A. Hudson, Douglas A. Mitchell, Michael F. Freeman

The paper updates a genome mining tool to identify borosin precursor peptides and characterizes new biosynthetic features.

ACS Chemical Biology · May 2, 2024Peer-reviewedBioinformatics