Joachim Goedhart

The paper describes protocols for calcium imaging using a genetically encoded biosensor and fluorescence lifetime imaging to improve measurement robustness.

The paper systematically compares novel fluorescent proteins in budding yeast to evaluate their characteristics for research applications.

The paper develops a glucose biosensor, TINGL, for measuring intracellular glucose dynamics in yeast and human cells, showing it detects glucose concentrations up to 1 mM and responds to glucose addition in starved cells.

The paper describes a new calcium biosensor, G-Ca-FLITS, that maintains high brightness in both calcium-bound and -unbound states, enabling more accurate and consistent measurements.

The paper develops a method to track G-protein and ERK activities in single cells using biosensors but finds challenges in obtaining robust FRET measurements.

The paper shows that neutrophils use endothelial membrane tunnels for paracellular extravasation, formed by membrane lifting and indentation without requiring PECAM-1 or VE-cadherin.

This study explores mScarlet-based red fluorescent proteins for developing a calcium indicator with fluorescence lifetime contrast, but finds that lifetime contrast is lost in mammalian cells compared to bacterial lysates.

The paper uses biosensors to study CaSR-mediated signaling pathways in HEK-293 cells, showing activation of Gq and Gi proteins and their effects on intracellular calcium and cAMP levels.

EzReverse is a web tool that adjusts the background of color images for better visualization, offering flexible inversion methods and multiple color space options.

The paper introduces SATTs to identify E3-specific SUMO substrates, finding 1681 SUMO substrates across eight E3s with distinct preferences.

PlotXpress is a webtool that normalizes and visualizes reporter expression data using internal controls and provides standardized, publication-grade plots.

VolcaNoseR is a web tool for creating, exploring, labeling, and sharing volcano plots from genome- and proteome-wide screens.

This study defines the sequential and cooperative assembly mechanism of the TCR complex and reveals how TFIIH is recruited to DNA damage-stalled RNAPIIo.

The paper shows that DRY mutants of H1R and AT1AR can activate G protein signaling, challenging the belief that they are tools for studying arrestin-independent signaling.

The paper investigates the role of β-arrestins in H1R signaling using live cell assays and shows β-arrestins contribute to desensitization and that ERK activation is primarily Gq-mediated.

The paper demonstrates real-time detection of Ca2+ and cAMP signaling from olfactory receptors expressed in HEK293TN cells using fluorescent biosensors.

The paper characterizes 27 fluorescent proteins in vivo in yeast, finding differences from in vitro data and showing how codon optimization affects their properties.

This study uses caged DAG to show that increasing DAG levels enhances PKCbetaII membrane relocation amplitude and duration.

The paper presents a VBA/Excel macro to calculate Cliff's delta effect size and 95% confidence intervals for two independent samples.