Theodorus W. J. Gadella

The paper describes a new calcium biosensor, G-Ca-FLITS, that maintains high brightness in both calcium-bound and -unbound states, enabling more accurate and consistent measurements.

This paper evaluates mScarlet3-H, a red fluorescent protein with lower brightness and fluorescence lifetime, showing its potential for lifetime-based pH sensing and cellular lifetime unmixing.

This study explores mScarlet-based red fluorescent proteins for developing a calcium indicator with fluorescence lifetime contrast, but finds that lifetime contrast is lost in mammalian cells compared to bacterial lysates.