Regional random mutagenesis driven by multiple sgRNAs and diverse on-target genome editing events to identify functionally important elements in non-coding regions
Kento Morimoto, Hayate Suzuki, Akihiro Kuno…·Seiya Mizuno
The paper introduces a novel method for functional analysis of non-coding regions by inducing random mutations in target genomic regions using CRISPR and transposase in mouse embryonic stem cells.