Akihiro Kuno

TSUMUGI: a platform for phenotype-driven gene network identification from comprehensive knockout mouse phenotyping data

TSUMUGI identifies gene networks driven by phenotypes using IMPC knockout mouse data to facilitate hypothesis generation for complex phenotypes.

KOnezumi-AID: A Software to Automate the Design of Multiplex Knockouts Using Target-AID

KOnezumi-AID automates gRNA design for Target-AID-mediated gene knockouts, enabling efficient and safe multiplex knockout strategies in mouse and human genomes.

Regional random mutagenesis driven by multiple sgRNAs and diverse on-target genome editing events to identify functionally important elements in non-coding regions

The paper introduces a novel method for functional analysis of non-coding regions by inducing random mutations in target genomic regions using CRISPR and transposase in mouse embryonic stem cells.

Universal method for generating knockout mice in multiple genetic backgrounds using zygote electroporation

This paper introduces a universal method for generating knockout mice in multiple inbred strains using zygote electroporation, enabling efficient phenotyping for gene function and disease research.

Multiplex genotyping method to validate the multiallelic genome editing outcomes using machine learning-assisted long-read sequencing

This paper introduces DAJIN, a machine learning-assisted long-read sequencing method for comprehensive multiplex genotyping of genome editing outcomes, including intended and unintended mutations.

EXOC1 regulates cell morphology of spermatogonia and spermatocytes in mice

This study identifies EXOC1's role in regulating pseudopod formation in spermatogonia and syncytium formation in spermatocytes in mice, highlighting its interaction with Rac1 and SNAP23/STX2.