Seiya Mizuno

A Col22a1-EGFP reporter reveals dynamic three-dimensional organization of the myotendinous junction

This study uses a Col22a1-EGFP reporter to visualize and analyze the dynamic three-dimensional structure of the myotendinous junction across development and in response to mechanical stress.

A patient-derived LMX1B variant causes tissue-specific manifestations of nail-patella syndrome in mice

This paper creates a mouse model with a patient-derived LMX1B variant, showing tissue-specific effects of nail-patella syndrome.

Continuous Nuclear Export of p62/SQSTM1 Is Essential for Kidney Homeostasis

This study shows that continuous nuclear export of p62 is essential for kidney homeostasis, as its disruption leads to renal failure due to excessive nuclear accumulation and aggregate formation.

Visualization of neuronal morphology of the suprachiasmatic nucleus vasopressin neurons by Cre/FLPe-based genetic two-step sparse labelling - GT-SPARCL

The paper introduces GT-SPARCL, a genetic method for low-density neuronal labeling in mice, and applies it to reveal morphological subtypes of AVP neurons in the SCN.

Tff2 marks gastric corpus progenitors that give rise to pyloric metaplasia/SPEM following injury

This study identifies Tff2+ progenitors in the gastric corpus as the primary source of SPEM and gastric cancer following injury, showing they can bypass metaplasia and progress directly to dysplasia.

TSUMUGI: a platform for phenotype-driven gene network identification from comprehensive knockout mouse phenotyping data

TSUMUGI identifies gene networks driven by phenotypes using IMPC knockout mouse data to facilitate hypothesis generation for complex phenotypes.

Early development of male germ cell clones shapes their reproductive success

The paper traces individual PGC clones in mice to show early stochastic bottlenecks shape clonal contributions and reproductive success.

Spatiotemporal dynamics of CD73 in mouse retina under physiological conditions

This study examines the spatiotemporal expression of CD73 in the mouse retina across development and adulthood, revealing its role in retinal function and adenosine signaling.

A novel genetic mouse model of fatal aortic dissection reveals massive inflammatory cell infiltration in the thoracic aorta

This paper introduces a novel genetic mouse model of aortic dissection showing immune cell infiltration and altered ECM dynamics.

Muc6-expressing gastric isthmus progenitors contribute to regeneration and metaplasia supported by myeloid-mesenchymal interactions

This study identifies Muc6-expressing isthmus progenitors as key contributors to gastric regeneration and metaplasia through myeloid-mesenchymal interactions.

KOnezumi-AID: A Software to Automate the Design of Multiplex Knockouts Using Target-AID

KOnezumi-AID automates gRNA design for Target-AID-mediated gene knockouts, enabling efficient and safe multiplex knockout strategies in mouse and human genomes.

Regional random mutagenesis driven by multiple sgRNAs and diverse on-target genome editing events to identify functionally important elements in non-coding regions

The paper introduces a novel method for functional analysis of non-coding regions by inducing random mutations in target genomic regions using CRISPR and transposase in mouse embryonic stem cells.

Universal method for generating knockout mice in multiple genetic backgrounds using zygote electroporation

This paper introduces a universal method for generating knockout mice in multiple inbred strains using zygote electroporation, enabling efficient phenotyping for gene function and disease research.

Ablation of Max expression induces meiotic onset in sexually undifferentiated germ cells

This study shows that Max gene disruption in germ cells leads to meiotic onset but results in apoptosis due to stalled meiotic progression.

Activity of estrogen receptor beta expressing neurons in the medial amygdala regulates preference towards receptive females in male mice.

This study identifies that MeApd-ERβ+ neurons regulate receptivity-based preference in male mice but not sex-based preference.

Engineered SMCHD1 and D4Z4 mutations reveal roles of D4Z4 heterochromatin disruption and feedforward DUX4 network activation in FSHD

The paper uses engineered human myoblasts to show that D4Z4 heterochromatin disruption and DUX4 network activation are key in FSHD.

Coordination chemogenetics for activation of GPCR-type glutamate receptors in brain tissue

This paper develops a chemogenetic method to selectively activate mGlu1 receptors in brain tissue using a palladium complex and a mutant receptor, demonstrating its role in synaptic plasticity.

Production of Offspring from Azoospermic Mice with Meiotic Failure: Precise Biparental Meiosis within Halved Oocytes

This study shows that reducing ooplasmic volume in oocytes improves meiotic accuracy and embryo development, enabling offspring production from azoospermic mice with spermatocyte arrest.

Multiplex genotyping method to validate the multiallelic genome editing outcomes using machine learning-assisted long-read sequencing

This paper introduces DAJIN, a machine learning-assisted long-read sequencing method for comprehensive multiplex genotyping of genome editing outcomes, including intended and unintended mutations.

Unraveling the roles of Mast4 in amelogenesis via regulating DLX3 and stem cell maintenance of mouse incisors

This study identifies MAST4's role in regulating DLX3 and stem cell maintenance in mouse incisor development, leading to Amelogenesis Imperfecta.

Transcriptome analysis for the development of cell-type specific labeling to study olfactory circuits

The paper identifies cell-type specific genes in olfactory bulb projection neurons using single-cell RNA-seq and validates them as Cre-driver markers for genetic labeling.

EXOC1 regulates cell morphology of spermatogonia and spermatocytes in mice

This study identifies EXOC1's role in regulating pseudopod formation in spermatogonia and syncytium formation in spermatocytes in mice, highlighting its interaction with Rac1 and SNAP23/STX2.