Mark P. Styczynski

The paper shows that timing of metabolomics-driven supplementation in E. coli-based cell-free systems affects protein expression, with optimal timing after the inflection point of metabolic activity.

This study examines plasmid crosstalk in cell-free biosensors and shows how it can be leveraged to improve biosensor performance.

This paper introduces a modular cell-free biosensing platform called TLISA for rapid, equipment-free protein detection using split T7 RNA polymerase and affinity domains.

The paper introduces three new constraint classes for LK-DFBA to improve metabolic modeling accuracy and shows that a single wild-type dataset can identify optimal constraints for a system.

This paper demonstrates the use of lysate-based cell-free biosensors integrated with personal glucose monitors for on-site analyte quantification.

This paper introduces a protocell array platform for simultaneous detection of diverse analytes, demonstrating its ability to measure small molecules, minerals, and nucleic acids from a single sample.

This study investigates metabolic dynamics in Escherichia coli-based cell-free systems using metabolomics, finding that lysate preparation significantly affects protein yield and metabolic profiles, while endogenous metabolism is resilient to enzymatic perturbations.

SCOUR predicts metabolite-dependent regulatory interactions in metabolic networks using a stepwise machine learning framework, achieving high accuracy in identifying single-metabolite-controlled reactions.

The paper introduces MetaboPAC, a computational framework that infers absolute metabolite concentrations from relative abundance data using mass balance and kinetic equations, outperforming existing methods under various conditions.

The paper presents a longitudinal multi-omics analysis of P. cynomolgi infection in Macaca mulatta, identifying distinct signatures of acute and relapsing malaria infections.

The paper introduces ElectroPen, a low-cost electroporator for delivering molecules across cell membranes, demonstrating comparable efficiency to commercial devices at a fraction of the cost.