Yan Zhang

The paper identifies nuclear N-glycosylation as a novel modification that influences cell identity and is linked to neurodevelopmental disorders.

This study optimizes the One-Pot PURE system by addressing expression instability and proteolysis in E. coli strains, improving protein production efficiency.

This paper demonstrates the use of lysate-based cell-free biosensors integrated with personal glucose monitors for on-site analyte quantification.

This paper introduces a protocell array platform for simultaneous detection of diverse analytes, demonstrating its ability to measure small molecules, minerals, and nucleic acids from a single sample.

This study investigates metabolic dynamics in Escherichia coli-based cell-free systems using metabolomics, finding that lysate preparation significantly affects protein yield and metabolic profiles, while endogenous metabolism is resilient to enzymatic perturbations.

The paper introduces ElectroPen, a low-cost electroporator for delivering molecules across cell membranes, demonstrating comparable efficiency to commercial devices at a fraction of the cost.

This study designs and validates a small RNA-based negative autoregulation network using computational modeling and cell-free systems, showing reduced response time and noise in gene expression.