Fernanda Piorino

The paper introduces a measurement framework to characterize cell-free expression systems by analyzing transcription and translation dynamics using nucleic acid templates and Escherichia coli extracts.

This study examines plasmid crosstalk in cell-free biosensors and shows how it can be leveraged to improve biosensor performance.

This study uses cell-free systems to characterize RNA circuits for programmable protein expression, logic, and biosensing, revealing design principles applicable across different environments.

This study evaluates the risks of AI-driven protein design by testing synthetic homologs against biosecurity screening software.

This study investigates metabolic dynamics in Escherichia coli-based cell-free systems using metabolomics, finding that lysate preparation significantly affects protein yield and metabolic profiles, while endogenous metabolism is resilient to enzymatic perturbations.