Defining bottlenecks and physiological impact of an orthogonal translation initiation system
Dominic Scopelliti, Russel M. Vincent, Andras Hutvagner, Fiona Whelan, William P. Klare, Ignatius Mayo Aquino Pang, George McDonald Church, Paul R. Jaschke
This study develops an orthogonal translation initiation system using an i-tRNA-AAC mutant and evaluates its physiological impact through fluorescence assays, proteomics, and tRNA sequencing.
bioRxiv · Dec 23, 2025 · PreprintSynthetic Biology