Russel M. Vincent

This study develops an orthogonal translation initiation system using an i-tRNA-AAC mutant and evaluates its physiological impact through fluorescence assays, proteomics, and tRNA sequencing.

This study identifies suitable tRNA sources for cell-free protein synthesis by evaluating tRNA pools from different E. coli strains and V. natriegens, finding that A19 tRNAs are most effective for in vitro translation.

The paper designs a biocontained phage system using amber stop codon suppression to control phage replication and prevent escape.

The paper measures the orthogonality of an engineered amber initiator tRNA in a genomically recoded E. coli strain and finds it increases reporter expression without off-target translation.