Pietro Ridone

This study uses ancestral sequence reconstruction to generate flagellin sequences and tests their ability to restore motility in E. coli and S. Typhimurium, finding limited sequence tolerance and species-specific differences.

The paper inserts fluorescent proteins near the plug domain of MotB to study proton flow regulation without affecting motility, but finds limited fluorescence and the need for further optimization.

This study classifies and characterizes MotAB stator complexes across bacterial phyla, identifying structural innovations linked to flagellar motility.

The paper engineers FliC chimeras from multiple bacterial species to restore motility in E. coli, finding that two chimeras containing the S. Typhimurium D2 domain successfully rescue motility.

The paper introduces pCASKD, a single-plasmid system for scarless gene editing in E. coli, demonstrating efficient knock-in and knock-out edits with reduced false positives and faster workflow.

The paper designs and tests chimeric ion-powered rotary motors (IRMs) by combining components from different bacterial systems, showing that functional improvements require substitutions outside the hybrid pore region.

This study uses droplet-hydrogel bilayers to simultaneously record electrical activity and visualize fluorescently labeled Piezo1 channels, demonstrating their insertion and activation.