Author

Afsar Ali Mian

Aga Khan UniversityORCID 0000-0002-1995-7553Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Molecular Biology· 2 Cell Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Assay:CRISPR screen· 2 Research Focus:gene regulatory network inference· 2 Organism:human· 2 Research Focus:differential expression· 1 Assay:DNA methylation· 1 Research Focus:drug response / drug discovery· 1 Organism:E. coli· 1

Frequent Co-Authors

Irfan HussainiD· 3 shared Ambrin FatimaiD· 2 shared Fawad Ur RehmaniD· 2 shared Hijab ZahraiD· 2 shared Kainaat Mumtaz· 2 shared Maliha Javed· 2 shared Muhammad Jameel· 2 shared Susheel Fatima· 2 shared

Activity by Year

2023-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 45 mutual co-author links

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3 shared papersMolecular Biology

Irfan HussainORCID

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Author profile →

3 papers

Recent·Most cited

Prime Editing Corrects the HBB Codon 8/9 (+G) Mutation in Patient-Derived Induced Pluripotent Stem Cells and Restores β-Globin Expression in iPSC-Derived Erythroid Cells

Irfan Hussain, Kainaat Mumtaz, Maliha Javed, Susheel Fatima, Hijab Zahra, Anwar Alam, Muhammad Jameel, Naveed Altaf Malik, Farhatullah Syed, Fawad Ur Rehman, Salman Kirmani, Ambrin Fatima, Afsar Ali Mian

This study uses prime editing to correct the HBB c.27dupG mutation in patient-derived iPSCs and restores β-globin expression in erythroid cells.

bioRxiv · Sep 24, 2026 · PreprintCell Biology

A Rapid, Cost-Effective Method for High-Yield DNA Purification from PCR Products and Agarose Gels.

Susheel Fatima, Maliha Javed, Hijab Zahra, Irfan Hussain, Afsar Ali Mian

The paper develops a cost-effective in-house DNA purification protocol for PCR products and agarose gels, showing improved clarity and purity compared to commercial kits.

bioRxiv · Nov 13, 2024 · PreprintMolecular Biology

Prioritization of The Zinc finger domain within BCL11A gene by the Amelioration capability of hemoglobinopathies using CRISPR-Cas9 technology

Kainaat Mumtaz, Irfan Hussain, Fizza Iftikhar, Fawad Ur Rehman, Muhammad Jameel, Safana Farooq, Hammad Hassan, Ambrin Fatima, Oliver Gerhard Ottmann, Afsar Ali Mian

This study uses CRISPR-Cas9 to investigate BCL11A zinc finger domains' role in gamma-globin regulation and hemoglobinopathy treatment, finding Znf4 deletion increases HbF levels.

bioRxiv · Nov 28, 2023 · PreprintMolecular Biology