Trinh Lam

Same-cell mapping of epigenetic regulatory state to functional proteomic output

Proteo-Seq links chromatin accessibility and proteomic output in the same cell, revealing new protein-defined states in breast cancer cells.

Deterministic DNA barcoding using vacuum-driven loading of free oligonucleotides to microwell arrays

This paper introduces a deterministic DNA barcoding method using vacuum-driven microfluidics to load oligonucleotides into microwells, achieving uniform barcoding with reduced reagent use and cross-contamination.

Single-cell Organelle Extraction with Cellular Indexing

The paper introduces VacTrap, a microfluidic device that isolates and spatially indexes single nuclei from mammalian cells with high efficiency, enabling single-cell multiomics applications.

EpiBlot: Joint Mapping of Chromatin Accessibility and Targeted Proteomics in HER2-expressing Breast Cancer Systems

EpiBlot maps chromatin accessibility and proteoform expression in HER2-expressing breast cancer cells, revealing distinct epigenetic and protein responses to therapies.

Paired Analyses of Nuclear Protein Targets and Genomic DNA by Single-Cell Western Blot and Single-Cell PCR

The paper introduces SplitBlot, a microfluidic single-cell assay that pairs genomic DNA analysis with nucleo-cytoplasmic protein measurements from the same cell.

Single-cell western blotting of cytoplasmic cytokeratin 8 proteoforms

This paper develops a single-cell western blot method integrated with differential detergent fractionation to detect CK8 proteoforms while preserving nuclear integrity in breast cancer cells.