Mark A. Knepper

Kinome-wide CRISPR/Cas9-knockout screening reveals critical protein kinases in vasopressin V2-receptor signaling

A kinome-wide CRISPR knockout screen in a mouse collecting duct Aqp2-GFP reporter line found 26 kinases shaping vasopressin-driven Aqp2 transcription, including Dyrk1a, Stk11 and TGF-β receptors.

GFP Reporter System Reveals Cell-to-Cell Variability in Aquaporin-2 Expression

The paper develops an Aqp2 reporter cell line using CRISPR/Cas9 to study Aqp2 expression variability, finding that cell cycle state influences Aqp2 expression heterogeneity.

CREB-family transcription factors and vasopressin-mediated regulation of Aqp2 gene transcription

This study shows that ATF1, CREB1, and CREM redundantly regulate Aqp2 gene transcription in response to vasopressin in mpkCCD cells.

Vasopressin-Dependent β-Catenin Phosphorylation at Ser552 and Branching Structure of Mouse Collecting Duct System

This study shows that mutating Ser552 in β-catenin in mice alters collecting duct branching without affecting differentiation, suggesting vasopressin-mediated phosphorylation at Ser552 influences CD development.

Circadian Gene Expression in Mouse Renal Proximal Tubule

The paper identifies circadian gene expression patterns in mouse renal proximal tubules and provides a web resource for these data.

Early Signaling Events in Renal Compensatory Hypertrophy Revealed by Multi-Omics

The paper identifies PPAR activation as a key driver of compensatory renal proximal tubule hypertrophy using multi-omic analyses in a mouse model.

Targeted single-cell RNA-seq identifies minority cell types of kidney distal nephron that regulate blood pressure and calcium balance

The paper identifies minority cell types in the kidney distal nephron using single-cell RNA-seq and creates a web-based data resource for these cells.

Phosphoproteomic Identification of Vasopressin-Regulated Protein Kinases in Collecting Duct Cells

The paper identifies vasopressin-regulated protein kinases in collecting duct cells using phosphoproteomic analysis and finds six downstream kinases account for most phosphorylation sites.

Protein kinase A catalytic-α and catalytic-β proteins have non-redundant functions

The paper identifies non-redundant functions of PKA-C and PKA-Cβ in renal collecting duct cells through phosphoproteomic analysis and shows distinct effects on AQP2 abundance.

RNA-Seq and Protein Mass Spectrometry in Microdissected Kidney Tubules Reveal Signaling Processes that Initiate Lithium-Induced Diabetes Insipidus

The paper uses RNA-Seq and protein mass spectrometry on microdissected kidney tubules to identify signaling pathways involved in lithium-induced diabetes insipidus, showing NF-κB activation and reduced aquaporin-2 expression.

Cell-Type Selective Markers Represented in Whole-Kidney RNA-Seq Data

The paper identifies cell-type selective markers in whole-kidney RNA-Seq data and shows that proximal tubule cells dominate mRNA expression, affecting interpretation of bulk and single-cell RNA-Seq.