Chung‐Lin Chou

A kinome-wide CRISPR knockout screen in a mouse collecting duct Aqp2-GFP reporter line found 26 kinases shaping vasopressin-driven Aqp2 transcription, including Dyrk1a, Stk11 and TGF-β receptors.

This study shows that ATF1, CREB1, and CREM redundantly regulate Aqp2 gene transcription in response to vasopressin in mpkCCD cells.

This study shows that mutating Ser552 in β-catenin in mice alters collecting duct branching without affecting differentiation, suggesting vasopressin-mediated phosphorylation at Ser552 influences CD development.

The paper identifies circadian gene expression patterns in mouse renal proximal tubules and provides a web resource for these data.

The paper identifies vasopressin-regulated protein kinases in collecting duct cells using phosphoproteomic analysis and finds six downstream kinases account for most phosphorylation sites.

The paper uses RNA-Seq and protein mass spectrometry on microdissected kidney tubules to identify signaling pathways involved in lithium-induced diabetes insipidus, showing NF-κB activation and reduced aquaporin-2 expression.

The paper identifies cell-type selective markers in whole-kidney RNA-Seq data and shows that proximal tubule cells dominate mRNA expression, affecting interpretation of bulk and single-cell RNA-Seq.