Adrián Rafael Murillo‐de‐Ozores

A kinome-wide CRISPR knockout screen in a mouse collecting duct Aqp2-GFP reporter line found 26 kinases shaping vasopressin-driven Aqp2 transcription, including Dyrk1a, Stk11 and TGF-β receptors.

The paper develops an Aqp2 reporter cell line using CRISPR/Cas9 to study Aqp2 expression variability, finding that cell cycle state influences Aqp2 expression heterogeneity.

This study shows that ATF1, CREB1, and CREM redundantly regulate Aqp2 gene transcription in response to vasopressin in mpkCCD cells.

This study shows that mutating Ser552 in β-catenin in mice alters collecting duct branching without affecting differentiation, suggesting vasopressin-mediated phosphorylation at Ser552 influences CD development.

This paper determines the crystal structure of KLHL3 in complex with a WNK3 peptide, revealing structural features and phosphorylation-dependent regulation of WNK3 binding.