Author

Huaqiang Fang

Chinese Academy of Medical Sciences & Peking Union Medical CollegeORCID 0000-0002-2375-859XUnclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Neuroscience· 2

Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Organism:mouse· 2 Research Focus:cell type annotation· 1 Research Focus:cell-cell communication· 1 Assay:CRISPR screen· 1 Research Focus:drug response / drug discovery· 1 Assay:imaging· 1 Research Focus:protein design· 1

Frequent Co-Authors

Alexei M. BygraveiD· 1 shared Heping ChengiD· 1 shared Jue ZhangiD· 1 shared Lifeng ZhangiD· 1 shared Richard C. JohnsoniD· 1 shared Richard H. RothiD· 1 shared Richard L. HuganiriD· 1 shared Shenghong JuiD· 1 shared

Activity by Year

2021-2023 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 21 mutual co-author links

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1 shared paperNeuroscience

Alexei M. BygraveORCID

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2 papers

Recent·Most cited

Imaging Microglia Surveillance during Sleep-wake Cycles in Freely Behaving Mice

Xiaochun Gu, Zhong Zhao, Xueli Chen, Lifeng Zhang, Huaqiang Fang, Ting Zhao, Shenghong Ju, Weizheng Gao, Xiaoyu Qian, Xianhua Wang, Jue Zhang, Heping Cheng

This study uses in vivo imaging to show microglia surveillance is coupled to sleep-wake cycles and stress, with norepinephrine signaling playing a key role.

bioRxiv · Feb 3, 2023 · PreprintNeuroscience

An Optimized CRISPR/Cas9 Approach for Precise Genome Editing in Neurons

Huaqiang Fang, Alexei M. Bygrave, Richard H. Roth, Richard C. Johnson, Richard L. Huganir

This paper introduces TKIT, a CRISPR/Cas9 method for precise genomic knock-in in neurons, achieving up to 42% efficiency in mouse neurons and demonstrating its use in rat neurons for visualizing endogenous proteins.

eLife · Mar 10, 2021Peer-reviewedNeuroscience