Richard L. Huganir

Optimizing the efficiency of assisted reproduction procedures in the common marmoset (Callithrix jacchus) improved IVF outcomes

This study optimizes IVF procedures in common marmosets by identifying factors like progesterone levels and stimulation duration that improve embryo development and implantation rates.

Analysis of sperm processing and cryopreservation procedures in the common marmoset (Callithrix jacchus) to reduce fragmentation and maximize total and progressive motility

The paper optimizes sperm processing and cryopreservation methods in common marmosets to improve motility and reduce DNA fragmentation for IVF applications.

Neuronal Hyperactivity Induces Tau Phosphorylation Selectively in Excitatory Pyramidal Neurons Which Contributes to GluA2 Regulation

This study shows that neuronal hyperactivity selectively increases tau phosphorylation at S202/T205 in excitatory hippocampal neurons, which reduces GluA2 surface expression and may contribute to Alzheimer's disease pathology.

Sex and experience dependent regulation of synaptic protein turnover

The paper identifies sex and experience-dependent regulation of synaptic protein turnover using SILAM in the hippocampus, finding sex-specific changes in ASD risk genes and experience-induced stabilization of PSD proteins.

Dynamic visualization of physiological CaMKII activity using sensitive FRET biosensors

The paper develops sensitive FRET biosensors to visualize CaMKII activity dynamics in living cells and demonstrates their use in cultured and primary cells, including neurons and cardiomyocytes.

Rescuing Neurodevelopmental Deficits in AMPA Receptor Gain-of-Function Mutant

This study identifies a gain-of-function mutation in GRIA1 causing neurodevelopmental deficits and shows that an antisense oligonucleotide can prevent pathology and behavioral issues.

Syngap1 and the development of murine neocortical progenitor cells

This study shows that Syngap1 absence or haploinsufficiency does not affect murine neocortical progenitor properties or output, suggesting species-specific or methodological differences in SYNGAP1's role in neurogenesis.

Dynamic extracellular interactions with AMPA receptors

The paper identifies 70 extracellular proteins interacting with AMPARs during synaptic plasticity, showing OBCAM and NTM regulate AMPAR mobility.

CRISPR-mediated transcriptional activation as a mutation-independent therapeutic strategy for SYNGAP1-related intellectual disability

This paper demonstrates that CRISPR-mediated transcriptional activation can upregulate SYNGAP1 in a mouse model of SRID and human neurons, rescuing deficits without targeting mutations.

A Space-Time Hidden Markov Model for In Vivo NanoScale Synaptic Plasticity Tracking

The paper introduces SynTrack, a novel algorithm for tracking synaptic dynamics in vivo, achieving high accuracy and scalability in longitudinal synapse tracking.

Single-cell long-read sequencing of the experience-induced transcriptome

This study uses single-cell long-read sequencing to profile experience-induced gene and isoform expression in mouse hippocampal cells, identifying 1,266 experience-variable isoforms.

Architecture, Activation, and Conformational Plasticity in the GluA4 AMPA Receptor

This study investigates the structural features of GluA4 AMPA receptors, revealing their unique architecture and conformational plasticity that influence ion channel function and disease relevance.

Dissociation of SYNGAP1 Enzymatic and Structural Roles: Intrinsic Excitability and Seizure Susceptibility

This study shows that SYNGAP1's structural role, not its enzymatic GAP activity, is critical for seizure susceptibility in heterozygous KO mice, while its GAP activity affects intrinsic excitability.

Automatic detection of fluorescently labeled synapses in volumetric in vivo imaging data

The paper develops a convolutional neural network to detect and segment fluorescently labeled synapses in vivo, enabling large-scale analysis of synapses in living mice.

Calcium-permeable AMPA receptors govern PV neuron feature selectivity

This study shows that calcium-permeable AMPA receptors (CP-AMPARs) in PV interneurons reduce their feature selectivity, and removing them increases selectivity in visual cortex and hippocampus.

Endosomal hyper-acidification via proton-activated chloride channel deletion in neurons impairs AMPA receptor endocytosis and LTD

This study shows that deletion of the proton-activated chloride channel in neurons leads to endosomal hyper-acidification, impairing AMPA receptor endocytosis and long-term depression.

Modulation of GABAAreceptor trafficking by WWC2 reveals class-specific mechanisms of synapse regulation by WWC family proteins

This study shows WWC2 regulates GABAAR trafficking at inhibitory synapses, distinct from its homolog KIBRA's role at excitatory synapses.

Experience-induced remodeling of the hippocampal post-synaptic proteome and phosphoproteome

This study identifies experience-induced changes in the hippocampal PSD proteome and phosphoproteome in mice following inhibitory avoidance training or immediate shock.

Context-dependent hyperactivity in syngap1a and syngap1b zebrafish autism models.

This study generates zebrafish models of SYNGAP1 disorder and shows that syngap1ab mutants exhibit context-dependent hyperactivity linked to altered arousal states.

Mouse models of SYNGAP1-related intellectual disability

This paper generates two knock-in mouse models with human SYNGAP1 mutations causing intellectual disability, showing reduced protein levels and recapitulating key disease features.

SynGAP regulates synaptic plasticity and cognition independent of its catalytic activity

This study shows SynGAP regulates synaptic plasticity and cognition through phase separation, not its catalytic activity.

Btbd11 is an inhibitory interneuron specific synaptic scaffolding protein that supports excitatory synapse structure and function

This study identifies Btbd11 as an inhibitory interneuron-specific protein that supports glutamatergic synapse structure and function, with implications for neural circuitry and behavior.

Cross-modality supervised image restoration enables nanoscale tracking of synaptic plasticity in living mice

The paper develops a deep learning image restoration method to enhance in vivo synaptic imaging resolution using ex vivo super-resolution techniques, enabling tracking of synaptic plasticity in living mice.

Engineered Adhesion Molecules Drive Synapse Organization

This study engineers novel adhesion molecules that drive synapse formation in cultured neurons by combining domains from bacterial proteins and synaptic cell-adhesion molecules.

Tagging active neurons by soma-targeted Cal-Light

This paper introduces ST-Cal-Light, a method to tag active neurons by converting somatic calcium signals into gene expression, enabling precise functional circuit analysis.

The miR-124-AMPAR pathway connects polygenic risks with behavioral changes shared between schizophrenia and bipolar disorder

This study identifies the miR-124-AMPAR pathway as a common mediator linking polygenic risks to shared behavioral changes in schizophrenia and bipolar disorder.

Brain-wide measurement of synaptic protein turnover reveals localized plasticity during learning

The paper develops DELTA, a method to measure brain-wide synaptic protein turnover at single-synapse resolution, revealing localized plasticity in hippocampal CA1 during learning.

Cell-specific regulation of gene expression using splicing-dependent frameshifting.

This paper introduces SLED, a splicing-based method to control gene expression in specific cell types, demonstrating its utility in targeting neurons, photoreceptors, and cancer cells.

KIBRA-PKCγ signaling pathway modulates memory performance in mice and humans

This study identifies the KIBRA-PKCγ signaling pathway as crucial for modulating memory performance in both mice and humans.

Visualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors

The paper develops tools to track synaptic plasticity in vivo by imaging endogenous AMPA receptors and generates a detailed spatiotemporal map of synaptic changes during behavior.