Author

Justin N. Applegate

University of WashingtonORCID 0000-0002-2508-9190Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Microbiology· 2

Signature Assays, Organisms & Methods

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Assay:proteomics· 2 Approach:biophysical modeling· 1 Research Focus:cell signaling & trafficking· 1 Organism:E. coli· 1 Approach:experimental assay· 1 Approach:genetic perturbation / CRISPR· 1 Organism:human· 1 Organism:mouse· 1

Frequent Co-Authors

Afredo Ruiz-RiveraiD· 2 shared Alexey J. MerziD· 2 shared Angela LisovskyiD· 2 shared Beth A. TraxleriD· 2 shared Emma Y.D. MilleriD· 2 shared Zoey R. LittiD· 2 shared Courtney K. EllisoniD· 1 shared Luther DavisiD· 1 shared

Activity by Year

2026-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 45 mutual co-author links

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2 shared papersMicrobiology

Afredo Ruiz-RiveraORCID

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2 papers

Recent·Most cited

β-strand complementation within tip initiation complexes licenses assembly of diverse type IV filaments

Taylor J. Ellison, Zoey R. Litt, Justin N. Applegate, Emma Y.D. Miller, Luther Davis, Angela Lisovsky, Afredo Ruiz-Rivera, Rittika Saha, Naveen Jasti, Stephanie E. McLaughlin, Beth A. Traxler, Courtney K. Ellison, Alexey J. Merz

This paper identifies a β-strand complementation mechanism used by PilC/PilY1 proteins to license type IV filament assembly across diverse systems.

Proceedings of the National Academy of Sciences · Aug 31, 2026Peer-reviewedMicrobiology

Expression of soluble Type IV Minor Pilins and isolation of a Neisseria gonorrhoeae PilI-PilJ subcomplex

Justin N. Applegate, Emma Y.D. Miller, Zoey R. Litt, Afredo Ruiz-Rivera, Angela Lisovsky, Beth A. Traxler, Alexey J. Merz

This paper describes a method to express and purify soluble type IV minor pilins from E. coli and identifies a heterodimeric interaction between PilI and PilJ in Neisseria gonorrhoeae.

bioRxiv · Feb 9, 2026 · PreprintMicrobiology