Susan J. Rosser

Super-resolution imaging of proteins inside live mammalian cells with mLIVE-PAINT

This study adapts the LIVE-PAINT method for live mammalian cells, demonstrating super-resolution imaging of two proteins in real time.

Biofoundry-scale DNA assembly validation using cost-effective high-throughput long read sequencing

The paper introduces a cost-effective high-throughput long-read sequencing protocol for validating DNA assembly fidelity in biofoundries using Oxford Nanopore technology.

Live-cell super-resolution imaging of actin using LifeAct-14 with a PAINT-based approach

The paper introduces direct-LIVE-PAINT, a method for nanoscopic imaging of protein structures in live cells, demonstrating its application to actin with sub-diffraction resolution.

ACtivE: Assembly and CRISPR-targeted in vivo Editing for Yeast Genome Engineering Using Minimum Reagents and Time

ACtivE enables rapid, cost-effective yeast genome editing by in vivo DNA assembly, achieving high integration efficiency without cloning or reagents.

Multiplexed activation in mammalian cells using dFnCas12a-VPR

This paper demonstrates the use of dFnCas12a-VPR for multiplexed gene activation in mammalian cells, showing enhanced activity of 5' crRNAs in arrays.

DNA Features Viewer, a sequence annotation formatting and plotting library for Python

DNA Features Viewer provides a Python library for customizable sequence annotation visualizations with optimized layout and readability.

DNA Chisel, a versatile sequence optimizer

DNA Chisel provides a flexible framework for DNA sequence optimization through Python scripts and Genbank annotations.

Control of ΦC31 integrase-mediated site-specific recombination by protein trans splicing

The paper describes a split-intein system for regulating site-specific recombination using protein trans-splicing, demonstrating its application as a reversible AND gate.