Author

James W. Bryson

University of CopenhagenORCID 0000-0002-5360-6300Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:benchmark study· 1 Research Focus:benchmarking· 1 Assay:bulk RNA-seq· 1 Assay:CRISPR screen· 1 Approach:experimental assay· 1 Organism:mouse· 1 Research Focus:perturbation response prediction· 1

Frequent Co-Authors

Jamie AuxillosiD· 2 shared Anila GeorgeiD· 1 shared Junyu CheniD· 1 shared Lena Bjørn Johansson· 1 shared Sarah RennieiD· 1 shared Susan J. RosseriD· 1 shared

Activity by Year

2021-2026 · peak 1/yr

Co-Author Collaboration Network

6 top collaborators · 11 mutual co-author links

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2 shared papersGenomics

Jamie AuxillosORCID

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2 papers

Recent·Most cited

Systematic benchmark of demultiplexing workflows for direct RNA sequencing

James W. Bryson, Lena Bjørn Johansson, Anila George, Junyu Chen, Jamie Auxillos, Sarah Rennie

This study benchmarks two demultiplexing tools for direct RNA sequencing using IVT spike-ins and species identity, showing their performance differences and the need for calibration.

bioRxiv · Oct 10, 2026 · PreprintGenomics

Multiplexed activation in mammalian cells using dFnCas12a-VPR

James W. Bryson, Jamie Auxillos, Susan J. Rosser

This paper demonstrates the use of dFnCas12a-VPR for multiplexed gene activation in mammalian cells, showing enhanced activity of 5' crRNAs in arrays.

Nucleic Acids Research · Nov 26, 2021Peer-reviewedSynthetic Biology