The paper identifies Gfa, a new fosfomycin resistance protein family found in integrons, and shows it confers resistance across multiple bacterial species.

bioRxiv · Oct 10, 2026 · PreprintMicrobiology

Mobile Integrons Encode Phage Defense Systems

Nicolas Kieffer, Alberto Hipólito, Laura Ortiz-Miravalles, Paula Blanco, Thomas Delobelle, Patricia Vizuete, Francisco Manuel Ojeda, Thomas Jové, Dukas Jurėnas, Meritxell García‐Quintanilla, André F. P. Carvalho, Pilar Domingo‐Calap, José Antonio Escudero

The paper identifies novel phage resistance systems encoded in mobile integrons and shows they can be combined for multi-phage and antibiotic resistance with variable fitness costs.

Science · May 8, 2025Peer-reviewedMicrobiology

Ecosystems Oxygen Deficiency and Selection of Antimicrobial Resistance Genes in a One Health Perspective

Laura Ortiz-Miravalles, Amalia Prieto, Nicolas Kieffer, Ester Vergara, Rafael Cantón, Álvaro San Millán, Fernando Baquero, Alberto Hipólito, José Antonio Escudero

This study investigates how oxygen levels affect antimicrobial resistance gene performance in Enterobacterales, showing significant variations in resistance under aerobic and anaerobic conditions.

The Science of The Total Environment · Apr 27, 2025Peer-reviewedMicrobiology

Identification of Novel FosX Family Determinants from Diverse Environmental Samples

Nicolas Kieffer, Maria-Elisabeth Böhm, Fanny Berglund, Nachiket Prakash Marathe, Michael Richard Gillings, D. G. Joakim Larsson

This study identifies four novel fosfomycin resistance genes from environmental samples, which belong to the FosX family and show resistance to phosphonoformate inhibition.

Journal of Global Antimicrobial Resistance · Dec 24, 2024Peer-reviewedMicrobiology