Targeted biallelic integration of an inducible Caspase 9 suicide gene for safer cellular therapies prevents development of drug-resistant escapees in human iPSCs
Stephanie Wunderlich, Alexandra Haase, Sylvia Merkert, Kirsten Jahn, Maximillian Deest, Silke Glage, Wilhelm Korte, Andreas Martens, Andreas Kirschning, André Zeug, Evgeni G. Ponimaskin, Gudrun Goehring, Mania Ackermann, Nico Lachmann, Thomas Moritz, , Ulrich Martin
This study introduces a biallelic inducible Caspase9 suicide gene system in hiPSCs to prevent drug-resistant escapees, showing no escapees in biallelic cells versus rare escapees in monoallelic cells.
bioRxiv · Sep 19, 2021 · PreprintMolecular Biology