Genome-wide A to G and C to T Mutations Induced by Functional TadA Variants in Escherichia coli
Hao Wang, Zhengxin Dong, Jingyi Shi, Lei Chen, Tao Sun, Weiwen Zhang
This paper develops a system for inducing A[->]G and C[->]T mutations in E. coli using fusion proteins of DnaG with TadA variants, significantly increasing mutation rates and enabling rapid adaptive evolution.
ACS Synthetic Biology · Jan 9, 2025Peer-reviewedSynthetic Biology