A high throughput flow cytometry assay for quantifying type 3 secretion system assembly in Salmonella
Jordan Scott Summers, Julie Ming Liang, Nolan W. Kennedy, Danielle Tullman‐Ercek
The paper develops a high throughput flow cytometry assay to quantify T3SS assembly in Salmonella by measuring SipD abundance, validated with SR-SIM microscopy, and demonstrates its utility in studying regulator impacts and PrgI variants.