Nolan W. Kennedy

Glycerol alone effects 1,3-propanediol production via the aerobic propanediol utilization pathway in Salmonella enterica

This study shows Salmonella enterica can produce 1,3-propanediol from crude glycerol using the propanediol utilization pathway without high cofactor concentrations.

Distribution of luxS and production of autoinducer-2 among gut Bacteroidales

This study investigates the distribution of luxS and AI-2 production in gut Bacteroidales, showing that many species produce AI-2 but do not respond to it.

Engineering the Self-assembly of Bacterial Microcompartment Shell Proteins via Charged Mutations

This study shows that altering charge on bacterial microcompartment shell proteins can control their self-assembly into tubular structures.

Process for Standardizing and Assessing the Parameters Governing MS2 Virus-Like Particle Reassembly around Nucleic Acid Cargo

This paper introduces a standardized framework to quantify MS2 VLP reassembly yields and identifies key parameters affecting reassembly efficiency.

Self-assembling protein materials with genetically programmable morphology and size

This study investigates how sequence variations in BMC-H proteins influence their self-assembly into different supramolecular structures using cell-free synthesis and microscopy.

Synthetic Rewiring of Virus-Like Particles via Circular Permutation Enables Modular Peptide Display and Protein Encapsulation

The paper demonstrates the use of circular permutation to engineer virus-like particles, enabling peptide display and protein encapsulation through a comprehensive library and cryo-EM validation.

A high throughput flow cytometry assay for quantifying type 3 secretion system assembly in Salmonella

The paper develops a high throughput flow cytometry assay to quantify T3SS assembly in Salmonella by measuring SipD abundance, validated with SR-SIM microscopy, and demonstrates its utility in studying regulator impacts and PrgI variants.

Signal sequences target enzymes and structural proteins to bacterial microcompartments and are critical for microcompartment formation

This study investigates the role of signal sequences in bacterial microcompartment (MCP) assembly, showing that structural protein signal sequences are critical for MCP formation and that their deletion causes defects similar to full gene knockout.

Characterization and Engineering of the Type 3 Secretion System Needle Monomer from Salmonella Through the Construction and Screening of a Comprehensive Mutagenesis Library

This paper constructs and screens a mutagenesis library of the PrgI protein to identify mutations that enhance T3SS secretion titer and establish design rules for engineering the secretion system.

Linking the Salmonella enterica 1,2-propanediol utilization bacterial microcompartment shell to the enzymatic core via the shell protein PduB

This study identifies PduB as the protein linking the 1,2-propanediol utilization MCP shell to its enzymatic core in Salmonella, showing shell and core assembly are separate processes.

Density-based binning of gene clusters to infer function or evolutionary history using GeneGrouper

GeneGrouper clusters gene clusters across taxa to identify functional or evolutionary patterns, revealing a novel pduN pseudogene in Salmonella enterica.

Apparent size and morphology of bacterial microcompartments varies with technique

The paper shows that different imaging techniques produce varying apparent sizes and morphologies of bacterial microcompartments from Salmonella enterica serovar Typhimurium LT2, and provides guidelines for sample preparation.