Characterization and Engineering of the Type 3 Secretion System Needle Monomer from Salmonella Through the Construction and Screening of a Comprehensive Mutagenesis Library
Lisa Ann Burdette, Samuel Alexander Leach, Nolan W. Kennedy, Bon C. Ikwuagwu, Jordan Scott Summers, Danielle Tullman‐Ercek
This paper constructs and screens a mutagenesis library of the PrgI protein to identify mutations that enhance T3SS secretion titer and establish design rules for engineering the secretion system.
mSphere · Aug 7, 2024Peer-reviewedBioengineering