Danielle Tullman‐Ercek

This study shows Salmonella enterica can produce 1,3-propanediol from crude glycerol using the propanediol utilization pathway without high cofactor concentrations.

This study shows that altering charge on bacterial microcompartment shell proteins can control their self-assembly into tubular structures.

The paper develops an assay to distinguish pseudotransduction from true transduction in EV-AAV vectors, revealing cell-type-specific effects of EV modifications on transduction efficiency.

This paper introduces a standardized framework to quantify MS2 VLP reassembly yields and identifies key parameters affecting reassembly efficiency.

This study investigates how sequence variations in BMC-H proteins influence their self-assembly into different supramolecular structures using cell-free synthesis and microscopy.

The paper demonstrates the use of circular permutation to engineer virus-like particles, enabling peptide display and protein encapsulation through a comprehensive library and cryo-EM validation.

The paper develops a high throughput flow cytometry assay to quantify T3SS assembly in Salmonella by measuring SipD abundance, validated with SR-SIM microscopy, and demonstrates its utility in studying regulator impacts and PrgI variants.

The paper shows that encapsulating specific enzymes in bacterial microcompartments shifts pathway flux toward the encapsulated pathway, providing quantitative evidence for their use in metabolic engineering.

The paper evaluates cofactor recycling and permeability in bacterial microcompartments using an in vitro assay and kinetic model of the Pdu system, showing benefits for pathway flux.

The paper develops a high-throughput assay using a de novo fluorescent reporter to measure secreted protein via the Salmonella T3SS, revealing regulatory and structural insights.

This study investigates the role of signal sequences in bacterial microcompartment (MCP) assembly, showing that structural protein signal sequences are critical for MCP formation and that their deletion causes defects similar to full gene knockout.

This paper constructs and screens a mutagenesis library of the PrgI protein to identify mutations that enhance T3SS secretion titer and establish design rules for engineering the secretion system.

This paper develops a constitutively active type III secretion system in Salmonella to secrete heterologous proteins without inducer reagents, achieving production titers comparable to inducer-based systems.

This study demonstrates that bacterial microcompartments chemotax towards their substrate and are protected from aggregation in a cell-free system.

This study identifies PduB as the protein linking the 1,2-propanediol utilization MCP shell to its enzymatic core in Salmonella, showing shell and core assembly are separate processes.

GeneGrouper clusters gene clusters across taxa to identify functional or evolutionary patterns, revealing a novel pduN pseudogene in Salmonella enterica.

The paper shows that different imaging techniques produce varying apparent sizes and morphologies of bacterial microcompartments from Salmonella enterica serovar Typhimurium LT2, and provides guidelines for sample preparation.

The paper introduces a new gene expression cassette called a riboregulated switchable feedback promoter (rSFP) to dynamically control gene expression in metabolic engineering, achieving higher titers in a Taxol precursor biosynthesis pathway.

The paper proposes a spatially resolved kinetic model to explore optimal spatial organization strategies for biosynthetic pathways and finds that different pathways require distinct strategies based on kinetic and biophysical factors.

The paper shows that modifying shell proteins of bacterial microcompartments can improve metabolite diffusion and enhance pathway function in synthetic organelles.