This study isolates and characterizes a lytic phage effective against carbapenem-resistant Klebsiella pneumoniae, showing strong biofilm disruption and stability.

Microbiology Spectrum · May 29, 2026Peer-reviewedMicrobiology

Dengue serotype-1 virus like particles induce antibody responses following HeLa cell expression

Smita Shrestha, Archana Maharjan, Roji Raut, Binod Manandhar, Binod Khadka, Ajit Poudel, Tek Raj Joshi, Dilip Chaurasia, Saugat R.C, Jarina Joshi, Rajani Malla, Lata Karki, Ram Prasad Aganja, Rajindra Napit, Krishna Das Manandhar

This study produces Dengue-1 virus-like particles in HeLa cells that induce strong antibody responses in mice, suggesting their potential as a vaccine candidate and serodiagnostic tool.

bioRxiv · Apr 10, 2026 · PreprintImmunology

Rapid Isolation and Genomic Characterization of Sewage-Derived Bacteriophages Targeting Multidrug-Resistant Avian Pathogenic Escherichia coli in Poultry

Rajindra Napit, Anupama Gurung, Ashok B. Chaudhary, Machchendra Thapa, Roji Raut, Ajit Poudel, Sajisha Prajapati, Dhiraj Puri, Dibesh B. Karmacharya

This study isolates and characterizes bacteriophages from sewage targeting multidrug-resistant E. coli in poultry, showing their potential as alternatives to antibiotics.

bioRxiv · Feb 16, 2026 · PreprintMicrobiology

Genomic sequencing and neutralizing serological profiles during acute dengue infection: A 2017 cohort study in Nepal

Sabita Prajapati, Annie Elong Ngono, Melanie Mc Cauley, Julia Timis, Srijan Lal Shrestha, Anup Bastola, Shrawan Kumar Mandal, Sanjay Yadav, Rajindra Napit, Meng Ling Moi, Montarop Yamabhai, October Michael Sessions, Sujan Shresta, Krishna Das Manandhar

The paper analyzes genomic sequences and serological profiles of dengue patients in Nepal to identify circulating serotypes and immune responses during the 2017 dengue season.

PLOS Global Public Health · Nov 13, 2024Peer-reviewedGenomics

Dengue Virus Surveillance in Nepal Yields the First On-Site Whole Genome Sequences of Isolates from the 2022 Outbreak

Rajindra Napit, Annie Elong Ngono, Kathie A. Mihindukulasuriya, Aunji Pradhan, Binod Khadka, Smita Shrestha, Lindsay Droit, Anne M. Paredes, Lata Karki, Ravindra Khatiwada, Mamata Tamang, Bimal Sharma Chalise, Manisha Rawal, Bimalesh Kumar Jha, David Wang, , Krishna Das Manandhar

The paper reports the first on-site whole genome sequencing of dengue virus isolates from Nepal's 2022 outbreak, identifying DENV1, 2, and 3 and linking them to sequences from India and China.

BMC Genomics · Oct 24, 2024Peer-reviewedGenomics

Phylogenetic analysis shows canine distemper virus outbreak in stray dogs possibly occurs through spillover from wild carnivore reservoirs

Prajwol Manandhar, Rajindra Napit, Saman Man Pradhan, Pragun G Rajbhandari, Jessie A. Moravek, Pranav R Joshi, Rima Shrestha, Dibesh B. Karmacharya

The paper uses phylogenetic analysis to show CDV in stray dogs in Nepal likely spills over from wild carnivore reservoirs, belonging to the Asia-5 lineage also found in other species.

Virology Journal · Jun 6, 2023Peer-reviewedGenetics

One Health Assessment of an Urban Temporary Settlement Reveals Gut Microbiome Serving as Antimicrobial Resistance Gene Reservoir

Rajindra Napit, Anupama Gurung, Ajit Poudel, Prajwol Manandhar, Ashok B. Chaudhary, Ajay Narayan Sharma, Samita Raut, Saman Man Pradhan, Jyotsna Joshi, Mathilde Poyet, Mathieu Groussin, Rajesh Man Rajbhandari, Dibesh B. Karmacharya

The study assesses AMR gene reservoirs in human, animal, and environmental samples from a Kathmandu temporary settlement using metagenomics and network analysis.

bioRxiv · Mar 15, 2023 · PreprintGenomics