The ribonucleoprotein-mediated CRISPR-Cas9 system induces recurrent Aire gene mutations in contrast to the nickase expression vector in murine in vitro or in vivo models
Pedro Paranhos Tanaka, Cíntia Júnia Monteiro, Max Jordan Duarte, Ernna D. Oliveira, Ana C Monteleone-Cassiano, Romário S. Mascarenhas, Mayara Cristina Vieira-Machado, Adriana Arruda Matos, Leticia Agrelli de Brito, Alina O. Oliveira, Thiago M. Cunha, Eduardo Antônio Donadi, Geraldo Aleixo Silva Passos
The paper compares CRISPR-Cas9 RNP and nickase systems for inducing Aire gene mutations in murine mTECs, finding recurrent mutations with RNP but not nickase.
bioRxiv · Oct 13, 2023 · PreprintMolecular Biology