Ilona Kamila Gaszek

Saturation mutagenesis map of generalist versus specialist adaptations of β-lactamase to novel antibiotics

This study maps how β-lactamase mutations confer resistance to different antibiotics, identifying generalist and specialist mutations with distinct mechanisms and positions.

Higher-order epistasis drives evolutionary unpredictability toward novel antibiotic resistance

This study constructs a comprehensive mutant library of TEM-1 beta-lactamase and shows that higher-order epistasis drives unpredictable evolution of antibiotic resistance to novel substrates.

The highly evolvable nature of the antibiotic efflux protein TolC limits use of phages and bacterial toxins as antibacterial agents

The study shows that the TolC protein in E. coli is highly evolvable, making it a target for resistance against bacteriophages and bacterial toxins.