Kelli A. Connolly

This paper describes a standardized method to replace T cell receptors in murine CD8 T cells using CRISPR-Cas9 and retroviral transduction, enabling efficient genetic manipulation for in vitro and in vivo studies.

This study uses single-cell and spatial profiling to show that inflammatory signals from macrophages and fibroblasts drive CD8 T cell exhaustion and resistance to immune checkpoint blockade in prostate cancer.

This study shows CD8 T cells eliminate neoantigen+ epithelial stem cells in the colon, preventing cancer, without causing immunopathology.