Nikhil S. Joshi

This paper describes a standardized method to replace T cell receptors in murine CD8 T cells using CRISPR-Cas9 and retroviral transduction, enabling efficient genetic manipulation for in vitro and in vivo studies.

This study identifies the role of mature dendritic cells type 1 in forming, maintaining, and functioning tertiary lymphoid structures in cancer.

This study uses single-cell and spatial profiling to show that inflammatory signals from macrophages and fibroblasts drive CD8 T cell exhaustion and resistance to immune checkpoint blockade in prostate cancer.

The paper shows that humanized Traf6 promoter sequences in mice affect immune responses but not fertility, revealing distinct roles for germ and immune cells in gene regulatory evolution.

This study shows CD8 T cells eliminate neoantigen+ epithelial stem cells in the colon, preventing cancer, without causing immunopathology.

This paper introduces GSPA, a method to map gene space at single-cell resolution, enabling gene characterization and analysis of gene expression patterns across biological tasks.