John Attanasio

This paper describes a standardized method to replace T cell receptors in murine CD8 T cells using CRISPR-Cas9 and retroviral transduction, enabling efficient genetic manipulation for in vitro and in vivo studies.

The paper shows that humanized Traf6 promoter sequences in mice affect immune responses but not fertility, revealing distinct roles for germ and immune cells in gene regulatory evolution.

This study shows CD8 T cells eliminate neoantigen+ epithelial stem cells in the colon, preventing cancer, without causing immunopathology.

This paper introduces GSPA, a method to map gene space at single-cell resolution, enabling gene characterization and analysis of gene expression patterns across biological tasks.

This study identifies Pcdhg as a suppressor of Klf2/4 in endothelial cells, promoting atherosclerosis by inhibiting the Notch pathway.