Catharine Aquino

This study evaluates how PCR duplication affects RNA-seq data quality across four sequencers, finding that input amount and PCR cycles significantly impact duplication rates, with UMIs effectively reducing duplicates without losing biological information.

The paper compares scRNA-seq platforms and methods using SUM149PT cells treated with TSA, highlighting technical challenges and best practices for low RNA profiling.

HiDRA-seq enables high-throughput, low-cost SARS-CoV-2 testing using barcoded cDNA and NGS, allowing thousands of samples to be sequenced per run.