Hubert Rehrauer

This study evaluates how PCR duplication affects RNA-seq data quality across four sequencers, finding that input amount and PCR cycles significantly impact duplication rates, with UMIs effectively reducing duplicates without losing biological information.

This study uses single-cell long-read sequencing to analyze transcriptomes of ccRCC organoids, identifying novel transcripts and highlighting cell-specific alternative splicing events.

The paper performs a multiomic analysis of ALS patients and mouse models to identify sex differences, molecular subclusters, and the MAPK pathway as a therapeutic target.

This study compares human iPSC-derived inner ear organoids with early embryonic inner ear tissue, showing they recapitulate key developmental stages and marker expression.

The paper compares scRNA-seq platforms and methods using SUM149PT cells treated with TSA, highlighting technical challenges and best practices for low RNA profiling.

The paper constructs a molecular atlas of the human brain vasculature using single-cell RNA sequencing, revealing molecular heterogeneity and disease-related alterations in vascular cells.

The paper shows that longer DNA fragments in Illumina paired-end sequencing lead to lower base quality and higher error rates in the second read.