John M. Ashton

Reconstruction of septin higher-order nano-size structures in ovarian cancer cells uncover susceptibility to the septin-targeting small molecule UR214-9

This paper demonstrates that UR214-9 disrupts septin hetero-octamerization, leading to aberrant structures and impairing cancer cell functions.

Epicardial contributions to fibro-inflammatory signaling in a Pkp2-deficient arrhythmogenic cardiomyopathy model

This study shows that Pkp2 deficiency in epicardium-derived cells leads to fibro-inflammatory signaling and contributes to arrhythmogenic cardiomyopathy pathogenesis.

An assessment of normalization and differential expression methods for miRNA-seq analysis using a realistic benchmark dataset

The paper benchmarks normalization and differential expression methods for miRNA-seq using a realistic dataset, finding RPM normalization and edgeR perform best.

The impact of PCR duplication on RNA-seq data generated using NovaSeq 6000, NovaSeq X, AVITI and G4 sequencers.

This study evaluates how PCR duplication affects RNA-seq data quality across four sequencers, finding that input amount and PCR cycles significantly impact duplication rates, with UMIs effectively reducing duplicates without losing biological information.

Temporal Single Cell Analysis of Leukemia Microenvironment Identifies Taurine-Taurine Transporter Axis as a Key Regulator of Myeloid Leukemia

This study identifies the taurine-taurine transporter axis as a critical regulator of myeloid leukemia using temporal single-cell RNA-sequencing and functional experiments.

IL-1β/IRAK4 Axis Promotes Ovarian Tumor Development at the Mesothelium Injury Sites

This paper develops a MIM model to study EOC metastasis at mesothelial injury sites and shows IRAK4 inhibitors reduce tumor burden more effectively than IL-1/IL-1R1 targeting agents.

Single cell transcriptomic profiling identifies molecular phenotypes of newborn human lung cells

The paper uses single-cell RNA sequencing to create a molecular map of newborn human lung cells and identifies cell type-specific developmental states and biomarkers.

Single cell RNA sequencing analysis of mouse cochlear supporting cell transcriptomes with activated ERBB2 receptor, a candidate mediator of hearing restoration mechanisms.

The paper analyzes single-cell RNA sequencing data from mouse cochlear supporting cells with activated ERBB2 to identify gene expression changes associated with potential hearing restoration mechanisms.

Comparative analysis of single-cell RNA Sequencing Platforms and Methods

The paper compares scRNA-seq platforms and methods using SUM149PT cells treated with TSA, highlighting technical challenges and best practices for low RNA profiling.