Binding to Albumin and Off-Target Toxicity Confound the Use of LRRC8/VRAC Channel Blockers in Cell Physiology Assays
Marianne Touma Boulos, Aayan M. Afghan, Alena Rudkouskaya, Antonio M. Fidaleo, Harmaan S. Sidhu, Meraj Khan, Alexander A. Mongin
This study shows that VRAC blockers have off-target effects and cytotoxicity masked by serum albumin, suggesting they are not reliable for studying VRAC function.
bioRxiv · Jul 19, 2026 · PreprintPharmacology & Toxicology