Mafalda R. Almeida

This study shows that m6A modification on Xist RNA promotes its turnover, which regulates XCI dynamics, and that the NEXT complex mediates this turnover independently of YTHDC1.

The paper identifies YY1 binding as a gene-intrinsic barrier to Xist-mediated silencing, showing YY1 eviction is delayed during XCI.

The paper shows SPEN and Polycomb work together in parallel for Xist-mediated gene silencing, and SmcHD1 recruitment is needed for silencing X-linked genes during differentiation.

The paper shows that m6A modification affects alternative intron/exon inclusion in nascent RNA by disrupting splicing when METTL3 is depleted.

The paper systematically analyzes Xist-mediated allelic silencing in ES cells, identifying key factors and their roles in gene silencing during X chromosome inactivation.