Guifeng Wei

A contact-mediated transfer mechanism directs in cis localization and spread of Xist RNA

The paper identifies a contact-mediated transfer mechanism for Xist RNA localization and spread across the X chromosome.

A key role for chromosome compartment interactions in directing Xist RNA localisation

This study shows Xist RNA localizes to A1-sub-compartments on the X chromosome, which are positioned peripherally in compacted chromosome territories.

Selective interaction of the protein SMCHD1 with specific chromatin regions is governed by the loading factor LRIF1 and SMCHD1 ATPase activity

This study investigates SMCHD1's chromatin interactions and shows LRIF1 and ATPase activity are critical for its selective binding and epigenetic silencing functions.

N6-methyladenosine and the NEXT complex direct Xist RNA turnover and X inactivation dynamics

This study shows that m6A modification on Xist RNA promotes its turnover, which regulates XCI dynamics, and that the NEXT complex mediates this turnover independently of YTHDC1.

Absolute quantitative and base-resolution sequencing reveals comprehensive landscape of pseudouridine across the human transcriptome

This paper introduces BACS, a method for single-base resolution pseudouridine quantification, and identifies {Psi} sites in human RNA and viral transcripts.

Xist-mediated silencing requires additive functions of SPEN and Polycomb together with differentiation-dependent recruitment of SmcHD1

The paper shows SPEN and Polycomb work together in parallel for Xist-mediated gene silencing, and SmcHD1 recruitment is needed for silencing X-linked genes during differentiation.

Acute depletion of METTL3 identifies a role for N6-methyladenosine in alternative intron/exon inclusion in the nascent transcriptome

The paper shows that m6A modification affects alternative intron/exon inclusion in nascent RNA by disrupting splicing when METTL3 is depleted.

Time-resolved structured illumination microscopy reveals key principles of Xist RNA spreading

The paper visualizes Xist RNA dynamics in single cells during X chromosome inactivation using time-resolved structured illumination microscopy.

Systematic Allelic Analysis Defines the Interplay of Key Pathways in X Chromosome Inactivation

The paper systematically analyzes Xist-mediated allelic silencing in ES cells, identifying key factors and their roles in gene silencing during X chromosome inactivation.

A simple and effective method to isolate germ nuclei from C. elegans for genomic assays.

This paper introduces a method to isolate high-purity germ nuclei from C. elegans for genomic analyses like ChIP-seq and RNA-seq, without requiring specialized strains or conditions.