Joseph S. Bowness

This study uses cryo-electron tomography to reveal the multiscale compaction and organisation of the inactive X chromosome, showing the role of histone H1 and acetylation in chromatin structure.

This study uses Perturb-seq to identify gene regulatory programs in HSPCs that link genetic regulators to cell biological processes and stem cell phenotypes.

This paper introduces PerturbSpace, a method combining CRISPR perturbations with spatially hashed single-cell multiomics to study gene function in tissue architecture.

The paper develops sc-lentiMPRA to analyze enhancer activity and specificity in single cells during differentiation, finding motif affinity influences TF response patterns.

The paper identifies YY1 binding as a gene-intrinsic barrier to Xist-mediated silencing, showing YY1 eviction is delayed during XCI.

The paper shows SPEN and Polycomb work together in parallel for Xist-mediated gene silencing, and SmcHD1 recruitment is needed for silencing X-linked genes during differentiation.

The paper shows that m6A modification affects alternative intron/exon inclusion in nascent RNA by disrupting splicing when METTL3 is depleted.

The paper visualizes Xist RNA dynamics in single cells during X chromosome inactivation using time-resolved structured illumination microscopy.

The paper systematically analyzes Xist-mediated allelic silencing in ES cells, identifying key factors and their roles in gene silencing during X chromosome inactivation.