Neil Brockdorff

Nuclear-BRITE reduces background noise in MS2-based live-cell imaging of nuclear RNAs without affecting their abundance or function.

The paper identifies a contact-mediated transfer mechanism for Xist RNA localization and spread across the X chromosome.

This study uses cryo-electron tomography to reveal the multiscale compaction and organisation of the inactive X chromosome, showing the role of histone H1 and acetylation in chromatin structure.

This study shows Xist RNA localizes to A1-sub-compartments on the X chromosome, which are positioned peripherally in compacted chromosome territories.

This study investigates SMCHD1's chromatin interactions and shows LRIF1 and ATPase activity are critical for its selective binding and epigenetic silencing functions.

This study shows that m6A modification on Xist RNA promotes its turnover, which regulates XCI dynamics, and that the NEXT complex mediates this turnover independently of YTHDC1.

This study identifies the PWWP-ADD and N-terminal domains of DNMT3B1 as critical for its specificity in developmentally regulated CpG island methylation using an in vitro embryonic stem cell model.

The paper identifies YY1 binding as a gene-intrinsic barrier to Xist-mediated silencing, showing YY1 eviction is delayed during XCI.

The paper shows SPEN and Polycomb work together in parallel for Xist-mediated gene silencing, and SmcHD1 recruitment is needed for silencing X-linked genes during differentiation.

The paper shows that m6A modification affects alternative intron/exon inclusion in nascent RNA by disrupting splicing when METTL3 is depleted.

The paper visualizes Xist RNA dynamics in single cells during X chromosome inactivation using time-resolved structured illumination microscopy.

The paper systematically analyzes Xist-mediated allelic silencing in ES cells, identifying key factors and their roles in gene silencing during X chromosome inactivation.

This study shows PCF11 is critical for vertebrate development by promoting transcription termination and preventing transcriptional interference.

This study shows that SmcHD1 prevents TAD formation on the inactive X chromosome by altering chromatin structure and epigenetic marks.